Chemical reagents, kits, columns, and other products used for DNA synthesis, and to subsequently run purification columns; includes genetic assembly systems.
EasyScript PlusTM Reverse Transcriptase is a novel recombinant reverse transcriptase that exhibits much higher efficiency in the first strand cDNA synthesis from RNA templates with secondary structures and high GC contents. The EasyScript PlusTM Reverse Transcriptase is engineered to work under high temperatures (50°C-55°C), which can further facilitate to resolve the secondary structures and high GC problems of RNA. Due to this feature, full-length cDNA can be synthesized from RNA templates that are up to 15 kb.
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EasyScript Plus™ cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates with secondary structure and high GC contents. The kit utilizes a special Reverse Transcriptase, the EasyScript Plus™, which is a novel recombinant reverse transcriptase that exhibits much higher efficiency in the first strand cDNA synthesis from RNA templates with secondary structures and high GC contents. The EasyScript PlusTM Reverse Transcriptase is engineered to work under high temperatures (50°C-55°C), which can further facilitate to resolve the secondary structures and high GC problems of RNA. Due to this feature, full-length cDNA can be synthesized from RNA templates that are up to 15 kb.
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The First-Strand cDNA Synthesis Kit is based on Reverse Transcriptase (Cat. No. K1071) to synthesize first-strand cDNA from Total RNA or Poly(A)+ RNA. Reverse Transcriptase is a new enzyme obtained through genetic engineering based on M-MLV (RNase H-) Reverse Transcriptase. In comparison, Reverse Transcriptase reduces RNase H activity and increases thermal stability. Reverse Transcriptase can withstand higher reaction temperatures and is suitable for reverse transcription of RNA templates with complex secondary structures. In addition, Reverse Transcriptase enhances the affinity to the template, suitable for the reverse transcription of a small number of templates and low copy genes, and can generate cDNA up to 12.3 kb.
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More